Activating Compound | Comment | Organism | Structure |
---|---|---|---|
light | promotes ATP hydrolysis | Spinacia oleracea | |
sulfite | 10 mM stimulates both proton uptake and ATP hydrolysis of Mg2+-ATPase activity of trypsin-treated thylakoids | Spinacia oleracea | |
Trypsin | elicits higher rates of ATPase activity, Mg2+-ATPase activity of trypsinized thylakoids is only partially inhibited by the uncouplers | Spinacia oleracea |
Application | Comment | Organism |
---|---|---|
additional information | cleavage of the gamma subunit of the ATP synthase by trypsin prevents inhibition of ATPase activity by the sigma subunit, but only partially overcomes Mg2+-ADP inhibition during assay | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | incubation prior to assay abolishes Mg2+-ATPase activity of reduced thylakoids, has no effect on Mg2+-ATPase activity of trypsinized thylakoids | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
thylakoid membrane | - |
Spinacia oleracea | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contributes to significant ATPase activity in trypsin-treated membranes, whereas Ca2+-ATPase activity of reduced thylakoids is very low | Spinacia oleracea | |
Mg2+ | - |
Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphate + H+ | - |
Spinacia oleracea | ATP + H2O | - |
r | |
ATP + H2O + H+/in | - |
Spinacia oleracea | ADP + phosphate + H+/out | - |
r |
Synonyms | Comment | Organism |
---|---|---|
chloroplast ATP synthase | - |
Spinacia oleracea |